To acquire tradition extract, a colony was transferred to 10 ml of TSB broth and incubated overnight at 37 °C. The micro organism have been then cultured in excessive quantity of TSB and incubated at 37 °C. The bacteria had been proliferated in TSB broth medium and after in a single day incubation, was streaked on the blood agar medium. •B. coagulans can reduce progress of C. perfringens in broth medium. The very best fee of progress inhibition was observed in L. monocytogenes. Examination of t...
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